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Longevity

MOTS-c

Also known as: Mitochondrial Open Reading Frame of 12S rRNA-c

A mitochondrial-derived peptide studied for metabolic regulation, insulin sensitivity, and exercise capacity.

What is MOTS-c?

MOTS-c is a 16-amino-acid peptide encoded within the mitochondrial 12S rRNA. It functions as a mitokine — a hormone-like signal from mitochondria — and has been studied for its roles in glucose handling, exercise endurance, and aging-related metabolic decline.

Mechanism of action

MOTS-c activates AMPK signaling, enhances folate-methionine cycle activity, and modulates nuclear gene expression in response to metabolic stress. It improves insulin sensitivity and promotes substrate utilization during exercise.

Researched benefits

  • Improved insulin sensitivity in animal and human pilot work
  • Enhanced exercise capacity in rodent models, even in older animals
  • Anti-inflammatory and anti-obesity signaling
  • Mitochondrial biogenesis support

Researched dosage

Most research protocols use 500010000 mcg administered 1× per day via subcutaneous injection. Research has used 5–10 mg subcutaneously 2–3× per week. Human pharmacokinetic data is limited.

A typical cycle runs 4 weeks on, then 2 weeks off before reassessment.

Use the calculator below to convert your target MOTS-c dose into exact insulin-syringe units.

Reconstitution & storage

A standard 10 mg vial reconstituted with 2 mL of bacteriostatic water yields 5000 mcg/mL. Lyophilized stable at 2–8°C. Refrigerate post-reconstitution.

Side effects & considerations

  • Limited human safety data
  • Possible mild fatigue or flushing
  • Injection-site irritation

MOTS-c stacks

  • Metabolic Optimization — paired with Semaglutide. Layered metabolic and mitochondrial signaling research. Investigators have considered pairing MOTS-c with GLP-1 agonists in metabolic syndrome models. Not a clinical regimen.

Selected research

  • The mitochondrial-derived peptide MOTS-cCell Metabolism, 2015
  • MOTS-c improves muscle insulin sensitivity in aged miceCell Reports, 2018

MOTS-c dosage calculator

Compute reconstitution, exact units to draw, and vials per cycle — pre-loaded with MOTS-c defaults.

Common research range for MOTS-c: 500010000 mcg, 1× daily, subcutaneous. Cycle: 4 weeks on / 2 off.

Educational use only. This calculator performs unit math on the values you provide and is not a substitute for medical advice. Verify every calculation independently.

Result

Concentration
5000 mcg / mL
Per syringe unit
50.00 mcg / unit
Draw to this mark
150.0 units (1.50 mL)
Exceeds 100 units — use less diluent or a larger syringe.
Dose, all units
7500 mcg · 7.500 mg
Doses per vial
1.3 doses
Days per vial
1.3 days
Vials needed for 4-week cycle
21 vials

Numbers are computed from your inputs. Verify all calculations independently. Educational use only.

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MOTS-c FAQ

What is a mitokine?+

A mitokine is a signaling peptide produced by mitochondria that communicates with the rest of the cell and other tissues. MOTS-c is one of the best-studied examples.

Is MOTS-c safe?+

Animal data is encouraging, but human safety data is limited. Most research-grade use follows short cycles with careful monitoring.

How often should MOTS-c be dosed?+

Research protocols often use 5–10 mg subcutaneously 2–3× per week, mirroring its hormonal signaling profile rather than a daily injectable.

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