What is TB-500?
TB-500 is a synthetic peptide based on a region of Thymosin Beta-4, a 43-amino-acid protein expressed throughout the body. Research has focused on its role in actin sequestration, cell migration, and tissue regeneration, with attention from sports-recovery and veterinary research communities.
Mechanism of action
TB-500 binds and sequesters G-actin, regulating cytoskeletal dynamics that govern cell migration and differentiation. This is thought to support angiogenesis, keratinocyte migration, and the recruitment of stem cells to injured sites.
Researched benefits
- Accelerated wound and soft-tissue repair in animal studies
- Improved muscle and tendon flexibility post-injury
- Cardiac repair signaling in rodent ischemia models
- Hair-follicle stem-cell migration in preclinical work
Researched dosage
Most research protocols use 2000–5000 mcg administered 1× per day via subcutaneous injection. Research protocols frequently use a loading phase of 2–5 mg, 2× per week for 4–6 weeks, followed by a maintenance phase.
A typical cycle runs 6 weeks on, then 4 weeks off before reassessment.
Use the calculator below to convert your target TB-500 dose into exact insulin-syringe units.
Reconstitution & storage
A standard 5 mg vial reconstituted with 2 mL of bacteriostatic water yields 2500 mcg/mL. Store lyophilized vials at 2–8°C. After reconstitution, refrigerate and use within 30 days.
Side effects & considerations
- Lightheadedness shortly after injection
- Mild lethargy during the first week
- Injection-site sensitivity
TB-500 stacks
- Wolverine Stack — paired with BPC-157. Comprehensive soft-tissue regeneration. Pairs systemic actin-driven repair (TB-500) with local angiogenic and gut-healing signals (BPC-157).
Selected research
- Thymosin beta-4: a multi-functional regenerative peptide — Expert Opinion on Biological Therapy, 2015
- TB-500 in cardiac repair — Annals of the New York Academy of Sciences, 2012