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How Much BAC Water for 10mg MOTS-c?

Quick answer: For a 10mg MOTS-c vial, adding 2 mL of bacteriostatic water gives a concentration of 5000 mcg/mL. At this concentration, a 250 mcg dose equals approximately 5.0 syringe units on a U-100 insulin syringe. Use the concentration table below to choose the volume that best suits your target dose.

Concentration Table — 10mg MOTS-c

All syringe unit values assume a standard U-100 insulin syringe (100 units = 1 mL).

BAC Water AddedConcentration250 mcg Dose500 mcg Dose
1 mL10,000 mcg/mL2.50 units5.00 units
2 mLcommon5,000 mcg/mL5.00 units10.0 units
3 mL3,333 mcg/mL7.50 units15.0 units
5 mL2,000 mcg/mL12.5 units25.0 units
10 mL1,000 mcg/mL25.0 units50.0 units

Which BAC Water Volume Should You Use?

  • 1 mL — highest concentration (10,000 mcg/mL); best when working with very small dose volumes and large-barrel syringes.
  • 2 mL — the most common choice for MOTS-c; balances concentration with easy-to-measure syringe markings.
  • 3 mL — useful when target doses fall on inconvenient unit marks at 2 mL.
  • 5 mL — lower concentration makes very small doses easier to measure precisely; larger final volume dilutes the peptide more.
  • 10 mL — typically used for large-vial peptides or high-dose protocols; dose volumes will be larger per injection.

Researchers generally select the volume that places their target dose near a clean tick mark on the syringe barrel to minimize measurement error.

Reconstitution Calculator — MOTS-c

Common research range for MOTS-c: 500010000 mcg, 1× daily, subcutaneous. Cycle: 4 weeks on / 2 off.

Educational use only. This calculator performs unit math on the values you provide and is not a substitute for medical advice. Verify every calculation independently.

Result

Concentration
5000 mcg / mL
Per syringe unit
50.00 mcg / unit
Draw to this mark
150.0 units (1.50 mL)
Exceeds 100 units — use less diluent or a larger syringe.
Dose, all units
7500 mcg · 7.500 mg
Doses per vial
1.3 doses
Days per vial
1.3 days
Vials needed for 4-week cycle
21 vials

Numbers are computed from your inputs. Verify all calculations independently. Educational use only.

Storage After Reconstitution

Lyophilized stable at 2–8°C. Refrigerate post-reconstitution.

Frequently Asked Questions

Frequently asked questions

How much BAC water should I add to a 10mg MOTS-c vial?+

Adding 2 mL of bacteriostatic water to a 10mg MOTS-c vial yields a concentration of 5000 mcg/mL — a common starting point that keeps dose volumes practical. Other volumes (1, 3, 5 mL) are also used depending on the desired concentration.

What concentration does 10mg MOTS-c produce at 2 mL BAC water?+

At 2 mL of BAC water, a 10mg vial produces 5000 mcg/mL. A 250 mcg dose at this concentration equals approximately 5.0 syringe units on a U-100 insulin syringe.

How long does reconstituted MOTS-c last after adding BAC water?+

Lyophilized stable at 2–8°C. Refrigerate post-reconstitution.

Can I use sterile water instead of BAC water for MOTS-c?+

Bacteriostatic water (BAC water) is preferred because the 0.9% benzyl alcohol preservative inhibits bacterial growth, extending the shelf life of the reconstituted peptide to roughly 30 days. Sterile water lacks this preservative; if used, the reconstituted solution should be used within 24 hours.

Other MOTS-c Reconstitution Guides